Antimicrobial activity of an abundant calcium-binding protein in the cytoplasm of human neutrophils. J Infect Dis 1991 Jan;163(1):187-92
Date
01/01/1991Pubmed ID
1984467DOI
10.1093/infdis/163.1.187Scopus ID
2-s2.0-0025965196 (requires institutional sign-in at Scopus site) 125 CitationsAbstract
Studies of experimental infections in animals indicate that phagocytic cells may sometimes control infective foci without actually ingesting or contacting the invading microorganisms. In the present study, an effective inhibitor of Candida albicans growth, previously detected in neutrophils cytoplasm and found to be released only after lysis of the cells, was identified as an abundant calcium-binding protein originally described in neutrophils as the L1 myelomonocytic antigen or the cystic fibrosis antigen. This substance was demonstrated also to have static activity against several other important human pathogens, including Aspergillus fumigatus, Staphylococcus aureus, and Escherichia coli. Growth of the various microorganisms was inhibited to considerably different degrees by the neutrophil protein, with the effects on S. aureus (the least responsive organism) being significantly enhance by addition of calcium to the medium. These findings suggest that after its release by the death of neutrophils at sites of tissue infection, this abundant calcium-binding protein could have a host defense function by controlling the growth of pathogenic microorganisms that escape being killed initially and would otherwise be free to proliferate.
Author List
Sohnle PG, Collins-Lech C, Wiessner JHAuthor
Peter G. Sohnle MS, MD Emeritus Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Aspergillus fumigatusBacteria
Calcium
Calcium-Binding Proteins
Candida albicans
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Fungi
Humans
Neutrophils
Staphylococcus aureus
