Charge state coalescence during electrospray ionization improves peptide identification by tandem mass spectrometry. J Am Soc Mass Spectrom 2012 Aug;23(8):1390-9
Date
05/23/2012Pubmed ID
22610994Pubmed Central ID
PMC6345509DOI
10.1007/s13361-012-0404-0Scopus ID
2-s2.0-84865770129 (requires institutional sign-in at Scopus site) 47 CitationsAbstract
We report the effects of supercharging reagents dimethyl sulphoxide (DMSO) and m-nitrobenzyl alcohol (m-NBA) applied to untargeted peptide identification, with special emphasis on non-tryptic peptides. Peptides generated from a mixture of five standard proteins digested with trypsin, elastase, or pepsin were separated with nanoflow liquid chromatography using mobile phases modified with either 5% DMSO or 0.1%m-NBA. Eluting peptides were ionized by online electrospray and sequenced by both CID and ETD using data-dependent MS/MS. Statistically significant improvements in peptide identifications were observed with DMSO co-solvent. In order to understand this observation, we assessed the effects of supercharging reagents on the chromatographic separation and the electrospray quality. The increase in identifications was not due to supercharging, which was greater for the 0.1%m-NBA co-solvent and not observed for the 5.0% DMSO co-solvent. The improved MS/MS efficiency using the DMSO modified mobile phase appeared to result from charge state coalescence.
Author List
Meyer JG, A Komives EMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceBenzyl Alcohols
Dimethyl Sulfoxide
Molecular Sequence Data
Pancreatic Elastase
Pepsin A
Peptide Fragments
Proteins
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Trypsin