Faculty Collaboration Database

Comparative analysis of adipose-derived mesenchymal stem cells isolated from abdominal and breast tissue. Aesthet Surg J 2013 Aug 01;33(6):888-98

Date

08/03/2013

Pubmed ID

23908304

DOI

10.1177/1090820X13496115

Scopus ID

2-s2.0-84881169259 (requires institutional sign-in at Scopus site)   31 Citations

Abstract

BACKGROUND: Adipose-derived mesenchymal stem cells (ADSC) may have a potential dual role in soft tissue augmentation by suppressing inflammation and promoting regeneration. Due to these properties, there is increasing interest in their potential use in autologous fat grafting, particularly to the breast.

OBJECTIVES: The authors isolate and compare ADSC derived from abdominal and breast tissues with a hypothesis that different adipose tissue sources may demonstrate different functional characteristics affecting outcomes in autologous cell transplantation in reconstructive and aesthetic surgery.

METHODS: Adipose-derived mesenchymal stem cells from abdominal and breast tissues were isolated and compared in terms of surface marker expression, differentiation capabilities, and both fibroblast growth factor (FGF) and receptor expression. Immunophenotype of macrophages was also investigated using cell surface markers following a 7-day co-culture period with ADSC.

RESULTS: Results showed similar cell surface phenotype and multilineage differentiation capabilities of ADSC derived from abdominal and breast tissues. Variations of FGF expression were demonstrated on reverse transcription polymerase chain reaction, with a significantly higher expression of FGF2 seen in breast ADSC. Following the 7-day co-culture period, increased expression of the anti-inflammatory surface marker CD206 was identified, with decreased CD16 and human leukocyte antigen-DR on macrophages co-cultured with ADSC compared with controls.

CONCLUSIONS: The data indicate similarities between ADSC derived from abdominal and breast tissues. Significant differences were seen, however, in the expression of FGF2, which is important in angiogenesis and wound healing. The results support the utility of ADSC in cell-based therapies such as autologous fat grafting.

Author List

Hanson SE, Kim J, Hematti P

Author

Peiman Hematti MD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Abdominal Fat
Antigens, CD
Biomarkers
Breast
Cell Communication
Cell Differentiation
Cell Lineage
Cell Separation
Cells, Cultured
Coculture Techniques
Female
Fibroblast Growth Factors
Flow Cytometry
Gene Expression Regulation
Humans
Immunophenotyping
Macrophages
Mesenchymal Stem Cell Transplantation
Phenotype
Receptors, Fibroblast Growth Factor
Reverse Transcriptase Polymerase Chain Reaction
Time Factors