Measuring tumor cycling hypoxia and angiogenesis using a side-firing fiber optic probe. J Biophotonics 2014 Jul;7(7):552-64
Date
12/18/2012Pubmed ID
23242854Pubmed Central ID
PMC3702687DOI
10.1002/jbio.201200187Scopus ID
2-s2.0-84903739092 (requires institutional sign-in at Scopus site) 17 CitationsAbstract
Hypoxia and angiogenesis can significantly influence the efficacy of cancer therapy and the behavior of surviving tumor cells. There is a growing demand for technologies to measure tumor hypoxia and angiogenesis temporally in vivo to enable advances in drug development and optimization. This paper reports the use of frequency-domain photon migration with a side-firing probe to quantify tumor oxygenation and hemoglobin concentrations in nude rats bearing human head/neck tumors administered with carbogen gas, cycling hypoxic gas or just room air. Significant increase (with carbogen gas breathing) or decrease (with hypoxic gas breathing) in tumor oxygenation was observed. The trend in tumor oxygenation during forced cycling hypoxia (CH) followed that of the blood oxygenation measured with a pulse oximeter. Natural CH was also observed in rats under room air. The studies demonstrated the potential of the technology for longitudinal monitoring of tumor CH during tumor growth or in response to therapy.
Author List
Yu B, Shah A, Wang B, Rajaram N, Wang Q, Ramanujam N, Palmer GM, Dewhirst MWAuthor
Bing Yu PH.D. Assistant Professor of Biomedical Engineering in the Biomedical Engineering department at Marquette UniversityMESH terms used to index this publication - Major topics in bold
AnimalsCell Hypoxia
Cell Line, Tumor
Equipment Design
Equipment Failure Analysis
Fiber Optic Technology
Head and Neck Neoplasms
Humans
Neovascularization, Pathologic
Oximetry
Oxygen
Oxygen Consumption
Rats
Rats, Nude
Refractometry
Reproducibility of Results
Sensitivity and Specificity
Transducers
