Fluorescence activated cell sorting (FACS) using RNAlater to minimize RNA degradation and perturbation of mRNA expression from cells involved in initial host microbe interactions. J Microbiol Methods 2007 Jul;70(1):205-8
Date
05/22/2007Pubmed ID
17512621DOI
10.1016/j.mimet.2007.03.022Scopus ID
2-s2.0-34250764675 (requires institutional sign-in at Scopus site) 19 CitationsAbstract
Host microbe interactions frequently involve specific cellular tropism. Accurate characterization of cells involved in these initial interactions is complicated by the response to the microbe. We describe a method utilizing RNAlater for Fluorescence Activated Cell Sorting of these critical cells that minimizes the downstream perturbation in the gene expression profile.
Author List
Nishimoto KP, Newkirk D, Hou S, Fruehauf J, Nelson ELAuthor
Fang Yao Stephen Hou PhD, MB(ASCP)QCYM, MLS(ASCPi) Clinical Assistant Professor in the Biomedical Sciences Laboratory Programs department at University of Wisconsin - MilwaukeeMESH terms used to index this publication - Major topics in bold
Cells, CulturedEncephalitis Virus, Venezuelan Equine
Flow Cytometry
Gene Expression Profiling
Humans
Leukocytes, Mononuclear
RNA Stability
RNA, Messenger
