A latex particle assay for platelet-associated IgG. Transfusion 1992;32(3):235-8
Date
03/01/1992Pubmed ID
1557805DOI
10.1046/j.1537-2995.1992.32392213807.xScopus ID
2-s2.0-0026549013 (requires institutional sign-in at Scopus site) 7 CitationsAbstract
A modified, sensitive, solid-phase assay for platelet-associated IgG is reported. Direct comparisons were made between a 125I monoclonal radioimmunoassay (RIA) and the polyclonal antibody latex particle assay. In 209 simultaneous comparisons with the RIA, the sensitivity of the latex test was 91 percent, specificity was 100 percent, and overall efficiency was 97 percent. Commencing with platelet-rich plasma, the direct latex particle test for platelet-bound IgG requires 45 minutes; 90 minutes are required to crossmatch one patient with 12 donors. The advantages of the latex assay are absence of radioactivity, stability of reagents, economy, speed, specificity, and sensitivity.
Author List
Ramos RR, Curtis BR, Chaplin HAuthor
Brian Curtis PhD Director in the Platelet & Neutrophil Immunology Laboratory department at BloodCenter of WisconsinMESH terms used to index this publication - Major topics in bold
Antibodies, MonoclonalBlood Platelets
Humans
Immunoglobulin G
Methods
Radioimmunoassay
Sensitivity and Specificity