Faculty Collaboration Database

Enhanced hematopoietic activity of a human granulocyte/macrophage colony-stimulating factor-interleukin 3 fusion protein. Proc Natl Acad Sci U S A 1991 Jul 01;88(13):5809-13

Date

07/01/1991

Pubmed ID

1829529

Pubmed Central ID

PMC51967

DOI

10.1073/pnas.88.13.5809

Scopus ID

2-s2.0-0025945651 (requires institutional sign-in at Scopus site)   145 Citations

Abstract

Granulocyte/macrophage colony-stimulating factor-interleukin 3 (GM-CSF-IL-3) fusion proteins were generated by construction of a plasmid in which the coding regions of human GM-CSF and IL-3 cDNAs were connected by a synthetic linker sequence followed by subsequent expression in yeast. Both GM-CSF-IL-3 and IL-3-GM-CSF fusion proteins were purified to homogeneity and shown to bind to cell-surface receptors through either their GM-CSF or IL-3 domains. The fusion proteins exhibited enhanced receptor affinity, proliferative activity, and hematopoietic colony-stimulating activity compared with either IL-3 and/or GM-CSF alone. This suggests that GM-CSF-IL-3 fusion proteins may hold future promise as therapeutic agents.

Author List

Curtis BM, Williams DE, Broxmeyer HE, Dunn J, Farrah T, Jeffery E, Clevenger W, deRoos P, Martin U, Friend D

Author

Brian Curtis PhD Director in the Platelet & Neutrophil Immunology Laboratory department at BloodCenter of Wisconsin

MESH terms used to index this publication - Major topics in bold

Base Sequence
Binding, Competitive
Cloning, Molecular
Colony-Forming Units Assay
Dose-Response Relationship, Drug
Granulocyte-Macrophage Colony-Stimulating Factor
Hematopoiesis
Humans
In Vitro Techniques
Interleukin-3
Molecular Sequence Data
Oligonucleotides
Protein Conformation
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
Receptors, Interleukin-3
Recombinant Fusion Proteins