Faculty Collaboration Database

Erythrocyte adhesion is modified by alterations in cellular tonicity and volume. Br J Haematol 2005 Nov;131(3):366-77

Date

10/18/2005

Pubmed ID

16225657

DOI

10.1111/j.1365-2141.2005.05767.x

Scopus ID

2-s2.0-33644806121 (requires institutional sign-in at Scopus site)   22 Citations

Abstract

We tested the hypothesis that dehydration-induced alterations in red blood cell (RBC) membrane organisation or composition contribute to sickle cell adhesion in sickle cell disease (SCD). To examine the role of RBC hydration in adhesion to the subendothelial matrix protein thrombospondin-1 (TSP), normal and sickle RBCs were incubated in buffers of varying tonicity and tested for adhesion to immobilised TSP under flow conditions. Sickle RBCs exhibited a decrease in TSP binding with increasing cell hydration (P<0.005), suggesting that cellular dehydration may contribute to TSP adhesion. Consistent with this hypothesis, normal RBCs showed an increase in TSP adhesion with increasing dehydration (P<0.01). Furthermore, increased TSP adhesion of normal RBCs could also be induced by isotonic dehydration using nystatin-sucrose buffers. Finally, TSP adhesion of both sickle RBCs and dehydrated normal RBCs was inhibited by the anionic polysaccharides, chondroitin sulphate A and high molecular weight dextran sulphate, but not by competitors of CD47-, band 3-, or RBC phosphatidylserine-mediated adhesion. More importantly, we found increased adhesion of nystatin-sucrose dehydrated normal mouse RBCs to kidney capillaries following re-infusion in vivo. In summary, these findings demonstrate that changes in hydration can significantly impact adhesion, causing normal erythrocytes to display adhesive properties similar to those of sickle cells and vice versa.

Author List

Wandersee NJ, Punzalan RC, Rettig MP, Kennedy MD, Pajewski NM, Sabina RL, Paul Scott J, Low PS, Hillery CA

Author

Rowena C. Punzalan MD Associate Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adolescent
Adult
Anemia, Sickle Cell
Animals
Capillaries
Cell Adhesion
Cells, Cultured
Child
Dehydration
Erythrocyte Membrane
Erythrocytes
Erythrocytes, Abnormal
Flow Cytometry
Humans
Kidney
Mice
Nystatin
Osmosis
Phosphatidylserines
Sucrose
Thrombospondin 1