Faculty Collaboration Database

Biphasic and regionally-restricted chemokine expression in the central nervous system in the Theiler's virus model of multiple sclerosis. J Neurovirol 2000 May;6 Suppl 1:S44-52

Date

06/29/2000

Pubmed ID

10871765

Scopus ID

2-s2.0-0034034717 (requires institutional sign-in at Scopus site)   34 Citations

Abstract

Intracerebral infection of susceptible strains of mice with Theiler's murine encephalomyelitis virus (TMEV) induces a biphasic disease characterized by acute polioencephalitis followed by chronic demyelination and viral persistence in the spinal cord white matter. There has been limited study of soluble mediators responsible for the initial recruitment of inflammatory cells into the gray matter, and the secondary influx into the white matter during infection with TMEV. We used sensitive and specific RT - PCR/dot blot hybridization assays to quantitate the relative levels of chemokine mRNA in the brains and spinal cords during the acute and chronic phases of TMEV infection in mice susceptible (B10.M, H-2f) and resistant (B10, H-2b) to virus-induced demyelination. TMEV infection resulted in robust expression of mRNA for IP-10, RANTES, and MCP-1, but not GRO-alpha, in brains and spinal cords in both strains of mice within 5 days. By day 21, virus was cleared, inflammation reduced, and expression of all three chemokines subsided to baseline levels in the brains and spinal cords of resistant mice, and the brains of susceptible mice. Chemokine expression was also reduced in the spinal cords of susceptible mice, corresponding to a shift in TMEV replication from the gray to the white matter. During the chronic, demyelinating phase of infection, there was a resurgence in IP-10, RANTES, and MCP-1 mRNA in spinal cords of susceptible B10.M mice. This study demonstrates the coordinated regulation and regionally restricted expression of chemokines in a biphasic disease of the central nervous system and provides greater understanding of the mechanism by which inflammation is established and maintained in the CNS.

Author List

Murray PD, Krivacic K, Chernosky A, Wei T, Ransohoff RM, Rodriguez M

Author

Paul D. Harker-Murray MD, PhD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Acute Disease
Animals
Brain
Chemokine CCL2
Chemokine CCL4
Chemokine CCL5
Chemokine CXCL1
Chemokine CXCL10
Chemokines
Chemokines, CXC
Chemotactic Factors
Chronic Disease
Disease Models, Animal
Growth Substances
Intercellular Signaling Peptides and Proteins
Macrophage Inflammatory Proteins
Mice
Mice, Inbred C57BL
Multiple Sclerosis
Nucleic Acid Hybridization
Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Species Specificity
Spinal Cord
Theilovirus
Virulence