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Concentration by centrifugation for gas exchange EPR oximetry measurements with loop-gap resonators. J Magn Reson 2005 Oct;176(2):244-8

Date

07/26/2005

Pubmed ID

16040261

DOI

10.1016/j.jmr.2005.06.011

Scopus ID

2-s2.0-25144497630   34 Citations

Abstract

Measurement of the bimolecular collision rate between a spin label and oxygen is conveniently carried out using a gas permeable plastic sample tube of small diameter that fits a loop-gap resonator. It is often desirable to concentrate the sample by centrifugation in order to improve the signal-to-noise ratio (SNR), but the deformable nature of small plastic sample tubes presents technical problems. Solutions to these problems are described. Two geometries were considered: (i) a methylpentene polymer, TPX, from Mitsui Chemicals, at X-band and (ii) Teflon tubing with 0.075 mm wall thickness at Q-band. Sample holders were fabricated from Delrin that fit the Eppendorf microcentrifuge tubes and support the sample capillaries. For TPX, pressure of the sealant at the end of the sample tube against the Delrin sample holder provided an adequate seal. For Teflon, the holder permitted introduction of water around the tube in order to equalize pressures across the sealant during centrifugation. Typically, the SNR was improved by a factor of five to eight. Oxygen accessibility applications in site-directed spin labeling studies are discussed.

Author List

Subczynski WK, Felix CC, Klug CS, Hyde JS

Authors

Candice S. Klug PhD Professor in the Biophysics department at Medical College of Wisconsin
Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Centrifugation
Electron Spin Resonance Spectroscopy
Equipment Design
Equipment Failure Analysis
Flow Injection Analysis
Gases
Microfluidics
Oximetry
Oxygen
Transducers