Concentration by centrifugation for gas exchange EPR oximetry measurements with loop-gap resonators. J Magn Reson 2005 Oct;176(2):244-8
Date
07/26/2005Pubmed ID
16040261DOI
10.1016/j.jmr.2005.06.011Scopus ID
2-s2.0-25144497630 (requires institutional sign-in at Scopus site) 38 CitationsAbstract
Measurement of the bimolecular collision rate between a spin label and oxygen is conveniently carried out using a gas permeable plastic sample tube of small diameter that fits a loop-gap resonator. It is often desirable to concentrate the sample by centrifugation in order to improve the signal-to-noise ratio (SNR), but the deformable nature of small plastic sample tubes presents technical problems. Solutions to these problems are described. Two geometries were considered: (i) a methylpentene polymer, TPX, from Mitsui Chemicals, at X-band and (ii) Teflon tubing with 0.075 mm wall thickness at Q-band. Sample holders were fabricated from Delrin that fit the Eppendorf microcentrifuge tubes and support the sample capillaries. For TPX, pressure of the sealant at the end of the sample tube against the Delrin sample holder provided an adequate seal. For Teflon, the holder permitted introduction of water around the tube in order to equalize pressures across the sealant during centrifugation. Typically, the SNR was improved by a factor of five to eight. Oxygen accessibility applications in site-directed spin labeling studies are discussed.
Author List
Subczynski WK, Felix CC, Klug CS, Hyde JSAuthors
Candice S. Klug PhD Professor in the Biophysics department at Medical College of WisconsinWitold K. Subczynski PhD Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
CentrifugationElectron Spin Resonance Spectroscopy
Equipment Design
Equipment Failure Analysis
Flow Injection Analysis
Gases
Microfluidics
Oximetry
Oxygen
Transducers
