Improved mitochondrial bioenergetics by anesthetic preconditioning during and after 2 hours of 27 degrees C ischemia in isolated hearts. J Cardiovasc Pharmacol 2005 Sep;46(3):280-7
Date
08/24/2005Pubmed ID
16116332DOI
10.1097/01.fjc.0000175238.18702.40Scopus ID
2-s2.0-24344458955 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
We examined if sevoflurane given before cold ischemia of intact hearts (anesthetic preconditioning, APC) affords additional protection by further improving mitochondrial energy balance and if this is abolished by a mitochondrial KATP blocker. NADH and FAD fluorescence was measured within the left ventricular wall of 5 groups of isolated guinea pig hearts: (1) hypothermia alone; (2) hypothermia+ischemia; (3) APC (4.1% sevoflurane)+cold ischemia; (4) 5-HD+cold ischemia, and (5) APC+5-HD+cold ischemia. Hearts were exposed to sevoflurane for 15 minutes followed by 15 minutes of washout at 37 degrees C before cooling, 2 hours of 27 degrees C ischemia, and 2 hours of 37 degrees C reperfusion. The KATP channel inhibitor 5-HD was perfused before and after sevoflurane. Ischemia caused a rapid increase in NADH and a decrease in FAD that waned over 2 hours. Warm reperfusion led to a decrease in NADH and an increase in FAD. APC attenuated the changes in NADH and FAD and further improved postischemic function and reduced infarct size. 5-HD blocked the cardioprotective effects of APC but not APC-induced alterations of NADH and FAD. Thus, APC improves redox balance and has additive cardioprotective effects with mild hypothermic ischemia. 5-HD blocks APC-induced cardioprotective effects but not improvements in mitochondrial bioenergetics. This suggests that mediation of protection by KATP channel opening during cold ischemia and reperfusion is downstream from the APC-induced improvement in redox state or that these changes in redox state are not attenuated by KATP channel antagonism.
Author List
An J, Camara AK, Riess ML, Rhodes SS, Varadarajan SG, Stowe DFAuthors
Amadou K. Camara PhD Professor in the Anesthesiology department at Medical College of WisconsinDavid F. Stowe MD, PhD Professor in the Anesthesiology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
ATP-Binding Cassette TransportersAnesthetics
Anesthetics, Inhalation
Animals
Blood Pressure
Coronary Circulation
Energy Metabolism
Flavin-Adenine Dinucleotide
Guinea Pigs
Heart Rate
In Vitro Techniques
Ischemic Preconditioning, Myocardial
KATP Channels
Methyl Ethers
Mitochondria, Heart
NAD
Oxidation-Reduction
Oxygen Consumption
Potassium Channels, Inwardly Rectifying
Reperfusion Injury
Ventricular Function, Left