Replication of the adeno-associated virus DNA termini in vitro. Intervirology 1987;27(3):138-47
Date
01/01/1987Pubmed ID
2822604DOI
10.1159/000149732Scopus ID
2-s2.0-0023259041 (requires institutional sign-in at Scopus site) 37 CitationsAbstract
The validity of the model proposed by Cavalier-Smith for the replication of linear, single-stranded DNA molecules was tested by using subgenomic DNA termini isolated from adeno-associated virus (AAV), a defective parvovirus. In purified DNA replication systems, the terminal hairpin structure of AAV DNA with a 3'-OH end was shown to prime the synthesis of the daughter DNA strand, which was covalently linked to the parental strand. This resulted in the synthesis of a duplex DNA structure with a terminal hairpin. The availability of such a hairpin structure should facilitate the isolation and characterization of the pivotal enzyme, the putative nickase, for the replication of AAV DNA.
Author List
Srivastava AAuthor
Renren Wen PhD Adjunct Associate Professor in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AutoradiographyDNA Replication
DNA, Viral
Dependovirus
Electrophoresis, Polyacrylamide Gel
Genes, Viral
HeLa Cells
Humans
Models, Biological
Virus Replication