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Effect of low-density lipoprotein on endothelial cell membrane fluidity and mononuclear cell attachment. Am J Physiol 1991 Jan;260(1 Pt 1):C43-9

Date

01/11/1991

Pubmed ID

1987780

DOI

10.1152/ajpcell.1991.260.1.C43

Scopus ID

2-s2.0-0026060001   57 Citations

Abstract

To determine the effects of prolonged low-density lipoprotein (LDL) exposure in vitro on cultured endothelial cell (EC) lipid dynamics and cellular function, human umbilical vein ECs were incubated in LDL concentrations [cholesterol (Chol) = 240 mg/dl] associated with the premature development of atherosclerosis. After 4 days of incubation, cells were examined for changes in cellular lipid composition and for membrane fluidity. Results indicate that LDL-EC have increased Chol content (control EC vs. LDL-EC = 22.4 +/- 5.26 vs. 38.9 +/- 0.24 nmol/10(6) cells, P less than 0.05) and cellular Chol-to-phospholipid ratio (0.61 +/- 0.10 vs. 1.21 +/- 0.10 mol/mol, P less than 0.05). Augmentation of EC Chol content was accompanied by a marked decrease in EC cellular membrane fluidity as assessed by fluorescence polarization (anisotropy, r values, 0.172 +/- 0.019 vs. 0.226 +/- 0.014, P less than 0.0001). LDL-induced changes in EC lipid dynamics were associated with enhanced EC binding of monocytes (P less than 0.05) and U937 cells (P less than 0.01). Both LDL-induced decreases in membrane fluidity and enhanced attachment of mononuclear cells were reversed to control levels following a 2-min incubation of LDL-EC with the membrane mobility agent, A2C. These data therefore suggest that LDL-induced modulations in lipid dynamics play an important role in perturbation of EC function.

Author List

Pritchard KA Jr, Schwarz SM, Medow MS, Stemerman MB

Author

Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Cell Adhesion
Cell Line
Cells, Cultured
Cholesterol
Cholesterol, LDL
Endothelium, Vascular
Humans
Lipid Metabolism
Lipoproteins, LDL
Membrane Fluidity
Phospholipids
Umbilical Veins
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a