Differentially expressed genes in the rat cochlear nucleus. Neuroscience 2006 Oct 27;142(3):753-68
Date
08/15/2006Pubmed ID
16905270DOI
10.1016/j.neuroscience.2006.06.060Scopus ID
2-s2.0-33749255787 (requires institutional sign-in at Scopus site) 16 CitationsAbstract
The cochlear nucleus is the first central pathway involved in the processing of peripheral auditory activity. The anterior ventral cochlear nucleus (AVCN), posterior ventral cochlear nucleus (PVCN) and dorsal cochlear nucleus (DCN) each contain predominant populations of neurons that have been well characterized regarding their morphological and electrophysiological properties. Little is known, however, of the underlying genetic factors that contribute to these properties and the initial steps in auditory processing. Serial analysis of gene expression (SAGE), supported by microarray experiments, was performed on each subdivision of the rat cochlear nucleus to identify genes that may sub-serve specialized roles in the central auditory system. Pair-wise comparisons between SAGE libraries from the AVCN, PVCN and DCN were correlated with microarray experiments to identify individual transcripts with significant differential expression. Twelve highly correlated genes were identified representing cytoskeletal, vesicular, metabolic and g-protein regulating proteins. Among these were Rgs4 which showed higher expression in the DCN, Sst and Cyp11b1 with very high expression in the AVCN and Calb2 with preferential expression in the PVCN. The differential expression of these genes was validated with real-time reverse transcriptase-polymerase chain reaction. These experiments provide a basis for understanding normal auditory processing on a molecular level and a template for investigating changes that may occur in the cochlear nucleus with hearing loss, the generation and percept of tinnitus, and central auditory processing disorders.
Author List
Friedland DR, Popper P, Eernisse R, Cioffi JAAuthor
David R. Friedland MD Associate Director, Director, Chief, Professor in the Otolaryngology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCalbindin 2
Cochlear Nucleus
Female
Gene Expression
Gene Expression Profiling
Gene Library
Oligonucleotide Array Sequence Analysis
RGS Proteins
RNA, Messenger
Rats
Reverse Transcriptase Polymerase Chain Reaction
S100 Calcium Binding Protein G
Steroid 11-beta-Hydroxylase