Merocyanine 540-sensitized photoinactivation of leukemia cells: effects of dose fractionation. Photochem Photobiol 1992 Oct;56(4):489-93
Date
10/01/1992Pubmed ID
1454878DOI
10.1111/j.1751-1097.1992.tb02192.xScopus ID
2-s2.0-0026934112 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
The differential sensitivity to merocyanine 540 (MC540)-sensitized photoirradiation of leukemia cells, selected solid tumor cells, and normal pluripotent hematopoietic stem cells has been successfully exploited for the extracorporeal purging of simulated autologous remission bone marrow grafts. In this communication, we compare the effects of fractionated vs continuous irradiation upon the MC540-sensitized photoinactivation of L1210 and K562 leukemia cells. Exposure to MC540 (15 micrograms/mL) and fractionated doses of white light inactivated fewer in vitro clonogenic cells than exposure to an equivalent dose of continuous irradiation, provided the irradiation doses were small (8.1-16.2 kJ/m2) and spaced 1-2 h apart. The dye-sensitized photoinactivation of leukemia cells was enhanced when cells were stored at 4 degrees C instead of 37 degrees C between irradiation periods, most likely in part because the cells were unable to repair sublethal photodynamic damages at the lower temperature. These data suggest that cells can recover from sublethal damage inflicted by the plasma membrane-active photosensitizer, MC540.
Author List
Qiu K, Sieber FAuthor
Fritz Sieber PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Survival
Dose-Response Relationship, Radiation
Humans
Kinetics
Leukemia L1210
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Light
Mice
Pyrimidinones
Radiation-Sensitizing Agents
Temperature
Time Factors
Tumor Cells, Cultured
