The solution structure of the forkhead box-O DNA binding domain of Brugia malayi DAF-16a. Proteins 2014 Dec;82(12):3490-6
Date
10/10/2014Pubmed ID
25297652Pubmed Central ID
PMC4809524DOI
10.1002/prot.24701Scopus ID
2-s2.0-84937596643 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. Here the solution structure of the forkhead DNA binding domain of Brugia malayi DAF-16a, a putative ortholog of Caenorhabditis elegans DAF-16, is reported. It is believed to be the first structure of a forkhead or winged helix domain from an invertebrate. C. elegans DAF-16 is involved in the insulin/IGF-I signaling pathway and helps control metabolism, longevity, and development. Conservation of sequence and structure with human FOXO proteins suggests that B. malayi DAF-16a is a member of the FOXO family of forkhead proteins.
Author List
Casper SK, Schoeller SJ, Zgoba DM, Phillips AJ, Morien TJ, Chaffee GR, Sackett PC, Peterson FC, Crossgrove K, Veldkamp CTAuthor
Francis C. Peterson PhD Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Binding Sites
Brugia malayi
Caenorhabditis elegans Proteins
Conserved Sequence
DNA
Electrophoretic Mobility Shift Assay
Forkhead Transcription Factors
Helminth Proteins
Models, Molecular
Molecular Sequence Data
Molecular Weight
Peptide Fragments
Protein Conformation
Protein Structure, Tertiary
Recombinant Proteins
Response Elements
Sequence Alignment
Solubility
