Corticotropin releasing factor mRNA and peptide levels are differentially regulated in the developing ovine brain. Brain Res Mol Brain Res 1994 Nov;27(1):103-10
Date
11/01/1994Pubmed ID
7877440DOI
10.1016/0169-328x(94)90190-2Scopus ID
2-s2.0-0027985727 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
The regulation of CRF mRNA and protein in the developing ovine brain has been studied to assess the hypothesis that CRF is differentially regulated in the hypothalamus (Hypo), hippocampal-amygdala complex (H & A), frontal cerebral cortex (FCC) and brainstem (BS). We used a quantitative RNase protection assay and radioimmunoassay to determine mRNA and peptide concentrations, respectively, from the last third of gestation until term (i.e., from 95 to 142 days gestation (dg); term approximately 145 days). The major findings from this study are: (1) Hypothalamic CRF mRNA was increased by 2-fold in 140-142 dg fetuses compared to 128-138 and 95-123 dg fetuses; P = 0.016. (2) In the hypothalamus of 140-142 dg fetuses, there was a 2.5-fold increase in CRF mRNA derived from polyadenylation at poly(A) sites 2, 3 or 4; P = 0.005. (3) In 128-138 dg fetuses, CRF mRNA in the frontal cortex was 2-fold higher than in the other brain regions during this time period; P = 0.008. (4) CRF peptide concentrations in the Hypo were 2.5-fold higher in 140-142 dg fetuses compared to 95-106 and 128-138 dg fetuses; P = 0.007. (5) CRF peptide concentrations in the frontal cortex were 5.5-fold higher in 140-142 dg fetuses compared to fetuses at 95-106 dg; P = 0.004. (6) CRF peptide concentrations in the H & A were 5-fold higher in 140-142 dg fetuses compared to 95-106 dg fetuses; P = 0.029. The results from the present study demonstrate for the first time that CRF mRNA and peptide are differentially regulated in a region-specific manner during development.
Author List
Keiger CJ, O'Steen WK, Brewer G, Sorci-Thomas M, Zehnder TJ, Rose JCAuthor
Mary Sorci Thomas PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AmygdalaAnimals
Base Sequence
Brain
Brain Stem
Corticotropin-Releasing Hormone
Fetal Proteins
Frontal Lobe
Gene Expression Regulation, Developmental
Gestational Age
Hypothalamus
Molecular Sequence Data
Organ Specificity
RNA, Messenger
Sheep