Measurement of natural-killer cell lytic activity of adenovirus-infected or adenovirus-transformed cells. Methods Mol Med 2007;131:213-9
Date
07/28/2007Pubmed ID
17656785DOI
10.1007/978-1-59745-277-9_15Scopus ID
2-s2.0-34548612599 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
Natural-killer (NK) cells are lymphocytes that do not express the CD3 T-cell receptor but do express the CD16 (FcgammaRIII) and CD56 (isoform of NCAM) in humans or NK1.1 antigen in certain strains of mice. NK cells display spontaneous lytic activity but do not exhibit immunological memory. NK cells are important mediators of antiviral and antitumor immunity. Standard NK cytolysis assays measure the ability of NK cells to kill certain target cells (tumor cells, virally infected cells) in short-term (usually 4-6 h) cytolysis assays. This chapter details the use of the NK cell cytolysis assay using polyclonal populations of human or rodent NK cells.
Author List
Routes JMMESH terms used to index this publication - Major topics in bold
AdenoviridaeAnimals
Cell Line, Transformed
Cell Transformation, Viral
Cells, Cultured
Humans
Killer Cells, Natural
Mice