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Agrochemical control of plant water use using engineered abscisic acid receptors. Nature 2015 Apr 23;520(7548):545-8

Date

02/06/2015

Pubmed ID

25652827

DOI

10.1038/nature14123

Scopus ID

2-s2.0-84928473532   100 Citations

Abstract

Rising temperatures and lessening fresh water supplies are threatening agricultural productivity and have motivated efforts to improve plant water use and drought tolerance. During water deficit, plants produce elevated levels of abscisic acid (ABA), which improves water consumption and stress tolerance by controlling guard cell aperture and other protective responses. One attractive strategy for controlling water use is to develop compounds that activate ABA receptors, but agonists approved for use have yet to be developed. In principle, an engineered ABA receptor that can be activated by an existing agrochemical could achieve this goal. Here we describe a variant of the ABA receptor PYRABACTIN RESISTANCE 1 (PYR1) that possesses nanomolar sensitivity to the agrochemical mandipropamid and demonstrate its efficacy for controlling ABA responses and drought tolerance in transgenic plants. Furthermore, crystallographic studies provide a mechanistic basis for its activity and demonstrate the relative ease with which the PYR1 ligand-binding pocket can be altered to accommodate new ligands. Thus, we have successfully repurposed an agrochemical for a new application using receptor engineering. We anticipate that this strategy will be applied to other plant receptors and represents a new avenue for crop improvement.

Author List

Park SY, Peterson FC, Mosquna A, Yao J, Volkman BF, Cutler SR

Authors

Francis C. Peterson PhD Professor in the Biochemistry department at Medical College of Wisconsin
Brian F. Volkman PhD Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Abscisic Acid
Acclimatization
Agrochemicals
Amides
Arabidopsis
Arabidopsis Proteins
Binding Sites
Carboxylic Acids
Crystallography, X-Ray
Droughts
Genetic Engineering
Genotype
Ligands
Lycopersicon esculentum
Membrane Transport Proteins
Models, Molecular
Plant Transpiration
Plants
Plants, Genetically Modified
Stress, Physiological
Structure-Activity Relationship
Water
jenkins-FCD Prod-461 7d7c6113fc1a2757d2947d29fae5861c878125ab