Effect of chloramphenicol on in vitro function of lymphocytes. J Infect Dis 1979 Feb;139(2):220-4
Date
02/01/1979Pubmed ID
438534DOI
10.1093/infdis/139.2.220Scopus ID
2-s2.0-0018741358 (requires institutional sign-in at Scopus site) 18 CitationsAbstract
The effect of chloramphenicol on the in vitro function of human peripheral blood lymphocytes was studied in assays of lymphocyte transformation and lymphokine production. When lymphocytes were stimulated by phytohemagglutinin, concanavalin A, or pokeweed mitogen in the presence of various concentrations of chloramphenicol, only minimal effects on blastogenesis were noted. However, suppression by chloramphenicol of blastogenesis induced by candida antigen or streptokinase-streptodornase was greater in magnitude and was dose-dependent; blastogenesis was suppressed to 25%--30% or normal levels by concentrations of chloramphenicol of 25--50 microgram/ml. Chloramphenicol had little effect on the production of the lymphokine leukocyte migration inhibition factor by lymphocytes stimulated either by candida antigen or by concanavalin A, whereas puromycin at a concentration of 5 microgram/ml significantly suppressed this response. Thus chloramphenicol appears to suppress antigen-induced lymphocyte blastogenesis significantly but not lymphokine production by stimulated lymphocytes.
Author List
DaMert GJ, Sohnle PGMESH terms used to index this publication - Major topics in bold
Antigens, FungalChloramphenicol
Concanavalin A
Humans
In Vitro Techniques
Lymphocyte Activation
Lymphocytes
Lymphokines
Phytohemagglutinins
Pokeweed Mitogens
Puromycin
Streptodornase and Streptokinase
