Cell-mediated immunity to Pityrosporum orbiculare in tinea versicolor. J Clin Invest 1978 Jul;62(1):45-53
Date
07/01/1978Pubmed ID
96143Pubmed Central ID
PMC371735DOI
10.1172/JCI109112Scopus ID
2-s2.0-0018148974 (requires institutional sign-in at Scopus site) 59 CitationsAbstract
Pityrosporum orbiculare, the presumed etiologic agent of tinea versicolor, was cultured in vitro and antigenic extracts prepared from the cultured organisms. Studies with lymphocytes from human cord blood and peripheral blood of guinea pigs demonstrated that such extracts were not mitogenic. Further studies in guinea pigs indicated that the animals could be sensitized by the injection of P. orbiculare extract in Freund's complete adjuvant and that this extract could elicit lymphocyte transformation and delayed skin test responses in sensitized animals. A group of 12 tinea versicolor patients and 15 normal subjects were studied in vitro for cell-mediated immunity to P. orbiculare extract. The majority of the subjects tested in both groups demonstrated positive lymphocyte transformation responses to this extract, as well as to standard mitogens and common microbial antigens. However, lymphocytes from tinea versicolor patients produced significantly less leukocyte migration inhibitory factor activity when stimulated by Candida albicans and P. orbiculare extracts than did lymphocytes from normal subjects. This was also true if only subjects with positive lymphocyte transformation responses to these antigens were considered. Leukocyte migration inhibitory factor responses to streptokinase/streptodornase were not significantly different between the two groups. Therefore, it appears that although both normal subjects and tinea versicolor patients demonstrate prior sensitization to antigens of P. orbiculare, the effector function of lymphocytes from most tinea versicolor patients appears to be impaired in that they produce subnormal amounts of the mediator leukocyte migration inhibitory factor when stimulated with antigenic extracts of this organism.
Author List
Sohnle PG, Collins-Lech CAuthor
Peter G. Sohnle MS, MD Emeritus Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAntigens
Candida albicans
Cell Migration Inhibition
Cross Reactions
Epidermophyton
Fetal Blood
Guinea Pigs
Humans
Immunity, Cellular
Immunodiffusion
Lymphocyte Activation
Malassezia
Mitogens
Skin Tests
Tinea Versicolor
Trichophyton
