Detecting and identifying volatile aldehydes as dinitrophenylhydrazones using gas chromatography mass spectrometry. Free Radic Biol Med 1995 Mar;18(3):553-7
Date
03/01/1995Pubmed ID
9101246DOI
10.1016/0891-5849(94)e0121-xScopus ID
2-s2.0-0028955197 (requires institutional sign-in at Scopus site) 27 CitationsAbstract
The detection of aldehydes has become an important measure of lipid oxidation in biological milieu. Aldehyde 2,4-dinitrophenylhydrazones are easily prepared and readily purified by HPLC and/or TLC and have proven useful for the detection of aldehydes. The lower limit of detection for dinitrophenylhydrazones was significantly reduced by using gas chromatography-mass spectrometric (GC-MS) techniques. Individual dinitrophenylhydrazones were readily separated by GC and detected by both positive and negative ion MS. The two major ions in negative ion spectra were the 182 m/z fragment ion and the molecular ion. Positive ion spectra showed strong ions corresponding to the protonated molecular ion and a protonated iminium ion. The greatest sensitivity was obtained with negative ion detection (10 pg per injection). However, more structural information was obtained from analysis of the positive ion spectra. Dinitrophenylhydrazones of hydroxyaldehydes, like 4-hydroxynonenal, were analyzed after converting the dinitrophenylhydrazones into trimethylsiloxylethers. GC-MS with negative ion detection was used to identify and quantitate the release of 4-hydroxynonenal by alveolar macrophages exposed to nitrogen dioxide.
Author List
Thomas MJ, Robison TW, Samuel M, Forman HJAuthor
Michael J. Thomas PhD Professor in the Pharmacology and Toxicology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AldehydesAnimals
Free Radicals
Gas Chromatography-Mass Spectrometry
Hydrazones
In Vitro Techniques
Lipid Peroxidation
Macrophages, Alveolar
Nitrogen Dioxide
Oxidative Stress
Rats
