Restoring susceptibility induced MRI signal loss in rat brain at 9.4 T: A step towards whole brain functional connectivity imaging. PLoS One 2015;10(4):e0119450
Date
04/07/2015Pubmed ID
25844644Pubmed Central ID
PMC4386820DOI
10.1371/journal.pone.0119450Scopus ID
2-s2.0-84926645905 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
The aural cavity magnetic susceptibility artifact leads to significant echo planar imaging (EPI) signal dropout in rat deep brain that limits acquisition of functional connectivity fcMRI data. In this study, we provide a method that recovers much of the EPI signal in deep brain. Needle puncture introduction of a liquid-phase fluorocarbon into the middle ear allows acquisition of rat fcMRI data without signal dropout. We demonstrate that with seeds chosen from previously unavailable areas, including the amygdala and the insular cortex, we are able to acquire large scale networks, including the limbic system. This tool allows EPI-based neuroscience and pharmaceutical research in rat brain using fcMRI that was previously not feasible.
Author List
Li R, Liu X, Sidabras JW, Paulson ES, Jesmanowicz A, Nencka AS, Hudetz AG, Hyde JSAuthors
Andrew S. Nencka PhD Director, Associate Professor in the Radiology department at Medical College of WisconsinEric Paulson PhD Associate Professor in the Radiation Oncology department at Medical College of Wisconsin
Jason W. Sidabras PhD Assistant Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsBrain
Brain Mapping
Ear, Middle
Echo-Planar Imaging
Fluorocarbons
Image Processing, Computer-Assisted
Radiography
Rats
