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Failure of intravenous or intracardiac delivery of mesenchymal stromal cells to improve outcomes after focal traumatic brain injury in the female rat. PLoS One 2015;10(5):e0126551

Date

05/07/2015

Pubmed ID

25946089

Pubmed Central ID

PMC4422703

DOI

10.1371/journal.pone.0126551

Scopus ID

2-s2.0-84929105130   9 Citations

Abstract

Mesenchymal stromal cells secrete a variety of anti-inflammatory factors and may provide a regenerative medicine option for the treatment of traumatic brain injury. The present study investigates the efficacy of multiple intravenous or intracardiac administrations of rat mesenchymal stromal cells or human mesenchymal stromal cells in female rats after controlled cortical impact by in vivo MRI, neurobehavior, and histopathology evaluation. Neither intravenous nor intracardiac administration of mesenchymal stromal cells derived from either rats or humans improved MRI measures of lesion volume or neurobehavioral outcome compared to saline treatment. Few mesenchymal stromal cells (<0.0005% of injected dose) were found within 3 days of last dosage at the site of injury after either delivery route, with no mesenchymal stromal cells being detectable in brain at 30 or 56 days post-injury. These findings suggest that non-autologous mesenchymal stromal cells therapy via intravenous or intracardiac administration is not a promising treatment after focal contusion traumatic brain injury in this female rodent model.

Author List

Turtzo LC, Budde MD, Dean DD, Gold EM, Lewis BK, Janes L, Lescher J, Coppola T, Yarnell A, Grunberg NE, Frank JA

Author

Matthew Budde PhD Associate Professor in the Neurosurgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Administration, Intravenous
Animals
Brain
Brain Injuries
Cardiac Catheters
Cell- and Tissue-Based Therapy
Cells, Cultured
Disease Models, Animal
Female
Humans
Image Processing, Computer-Assisted
Magnetic Resonance Imaging
Mesenchymal Stem Cell Transplantation
Rats
Rats, Wistar
Treatment Failure
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a