An electron paramagnetic resonance investigation of the oxygen dependence of the arterial-venous gradient of nitrosyl hemoglobin in blood circulation. Free Radic Biol Med 2007 Oct 15;43(8):1208-15
Date
09/15/2007Pubmed ID
17854716Pubmed Central ID
PMC2030993DOI
10.1016/j.freeradbiomed.2007.06.024Scopus ID
2-s2.0-34548506531 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
Whether there is a nitrosyl hemoglobin (HbNO) gradient between the venous and the arterial parts of the circulatory system is a very controversial issue in nitric oxide research. We have carefully evaluated the measurement of HbNO concentration in blood using EPR generated in vivo by the NO donor DEANO under various oxygen tensions. We found that the absolute concentrations of HbNO in venous and arterial blood were the same within experimental error, independent of hemoglobin saturation; only the ratios of 5-coordinate and 6-coordinate HbNO differed. The HbNO concentration increased when the oxygen concentration breathed by the rats decreased in a manner that was linear in hemoglobin saturation. These results do not support the existence of an arterial-venous gradient of HbNO under our experimental conditions.
Author List
Jiang J, Corbett J, Hogg N, Mason RPAuthor
Neil Hogg PhD Associate Dean, Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsArteries
Electron Spin Resonance Spectroscopy
Hemoglobins
Hydrazines
Male
Oxygen
Partial Pressure
Rats
Veins
