Percutaneous ultrasound guided implantation of VX2 for creation of a rabbit hepatic tumor model. PLoS One 2015;10(4):e0123888
Date
04/09/2015Pubmed ID
25853660Pubmed Central ID
PMC4390313DOI
10.1371/journal.pone.0123888Scopus ID
2-s2.0-84928902468 (requires institutional sign-in at Scopus site) 21 CitationsAbstract
Creation of a VX2 tumor model has traditionally required a laparotomy and surgical implantation of tumor fragments. Open surgical procedures are invasive and require long procedure times and recovery that can result in post-operative morbidity and mortality. The purpose of this study is to report the results of a percutaneous ultrasound guided method for creation of a VX2 model in rabbit livers. A total of 27 New Zealand white rabbits underwent a percutaneous ultrasound guided approach, where a VX2 tumor fragment was implanted in the liver. Magnetic resonance imaging was used to assess for tumor growth and necropsy was performed to determine rates of tract seeding and metastatic disease. Ultrasound guided tumor implantation was successful in all 27 rabbits. One rabbit died 2 days following the implantation procedure. Two rabbits had no tumors seen on follow-up imaging. Therefore, tumor development was seen in 24/26 (92%) rabbits. During the follow-up period, tract seeding was seen in 8% of rabbits and 38% had extra-hepatic metastatic disease. Therefore, percutaneous ultrasound guided tumor implantation safely provides reliable tumor growth for establishing hepatic VX2 tumors in a rabbit model with decreased rates of tract seeding, compared to previously reported methods.
Author List
White SB, Chen J, Gordon AC, Harris KR, Nicolai JR, West DL, Larson ACAuthor
Sarah B. White MD, MS, FSIR, FCIRSE Associate Dean, Vice Chair, Professor in the Radiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCarcinoma, Hepatocellular
Disease Models, Animal
Female
Liver
Liver Neoplasms, Experimental
Neoplasm Transplantation
Rabbits
Surgery, Computer-Assisted
Ultrasonography