Amplified Mechanically Gated Currents in Distinct Subsets of Myelinated Sensory Neurons following In Vivo Inflammation of Skin and Muscle. J Neurosci 2015 Jun 24;35(25):9456-62
Date
06/26/2015Pubmed ID
26109668Pubmed Central ID
PMC4478256DOI
10.1523/JNEUROSCI.0549-15.2015Scopus ID
2-s2.0-84933557705 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Primary afferents are sensitized to mechanical stimuli following in vivo inflammation, but whether sensitization of mechanically gated ion channels contributes to this phenomenon is unknown. Here we identified two populations of murine A fiber-type sensory neurons that display markedly different responses to focal mechanical stimuli of the membrane based on their expression of calcitonin gene-related peptide (CGRP). Following inflammation of the hindpaw, myelinated, CGRP-positive neurons projecting to the paw skin displayed elevated mechanical currents in response to mechanical stimuli. Conversely, muscle inflammation markedly amplified mechanical currents in myelinated, CGRP-negative neurons projecting to muscle. These data show, for the first time, that mechanically gated currents are amplified following in vivo tissue inflammation, and also suggest that mechanical sensitization can occur in myelinated neurons after inflammation.
Author List
Weyer AD, O'Hara CL, Stucky CLAuthor
Cheryl L. Stucky PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Action PotentialsAnimals
Calcitonin Gene-Related Peptide
Fluorescent Antibody Technique
Inflammation
Male
Mice
Muscle, Skeletal
Nerve Fibers, Myelinated
Neurons, Afferent
Patch-Clamp Techniques
Physical Stimulation
Skin