Medical College of Wisconsin
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Intracellular trafficking pathway of BK Virus in human renal proximal tubular epithelial cells. Virology 2008 Feb 20;371(2):336-49

Date

11/03/2007

Pubmed ID

17976677

Pubmed Central ID

PMC2674336

DOI

10.1016/j.virol.2007.09.030

Scopus ID

2-s2.0-38649102147 (requires institutional sign-in at Scopus site)   38 Citations

Abstract

Intracellular trafficking of BK Virus (BKV) in human renal proximal tubular epithelial cells (HRPTEC) is critical for BKV nephritis. However, the major trafficking components utilized by BKV remain unknown. Coincubation of HRPTEC with BKV and microtubule disrupting agents prevented BKV infection as detected by immunofluorescence and western blot analysis with antibodies which recognize BKV large T antigen. However, inhibition of a dynein, cellular motor protein, did not interfere with BKV infection in HRPTEC. A colocalization study of BKV with the markers of the endoplasmic reticulum (ER) and the Golgi apparatus (GA), indicated that BKV reached the ER from 6 to 10 h, while bypassing the GA or passing through the GA too transiently to be detected. This study contributes to the understanding of mechanisms of intracellular trafficking used by BKV in the infection of HRPTEC.

Author List

Moriyama T, Sorokin A

Author

Andrey Sorokin PhD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

BK Virus
Cells, Cultured
Endoplasmic Reticulum
Epithelial Cells
Golgi Apparatus
Humans
Kidney Tubules, Proximal
Kinetics
Microtubules
Time Factors
Virus Internalization