Selection of retrovirally transduced cells to enhance the efficiency of gene therapy. Proc Assoc Am Physicians 1997 Mar;109(2):111-9
Date
03/01/1997Pubmed ID
9069579Scopus ID
2-s2.0-0030899477 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Retrovirally mediated gene transfer is the approach of choice for many laboratories interested in gene therapy. One problem, however, is that the efficiency of therapeutic gene transfer to some important target cells is low. A strategy employed by a number of investigators to overcome this shortcoming is to construct recombinant viruses that employ selection modalities. These strategies can be classified broadly into either metabolic or cell-surface selection strategies. This article examines each selection approach and notes their important strengths and weaknesses. Further, the various gene products used in each approach to effect sorting of transduced cells is detailed.
Author List
Medin JA, Karlsson SAuthor
Jeffrey A. Medin PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, CD
Antigens, Surface
CD24 Antigen
Drug Resistance, Multiple
Gene Transfer Techniques
Genetic Therapy
Humans
Membrane Glycoproteins
Mice
Mutation
Retroviridae
Serum Albumin
Tetrahydrofolate Dehydrogenase
Transduction, Genetic
