Molecular interactions between interferon consensus sequence binding protein and members of the interferon regulatory factor family. Proc Natl Acad Sci U S A 1994 May 24;91(11):5046-50
Date
05/24/1994Pubmed ID
8197182Pubmed Central ID
PMC43928DOI
10.1073/pnas.91.11.5046Scopus ID
2-s2.0-0028229886 (requires institutional sign-in at Scopus site) 168 CitationsAbstract
Interferon (IFN) consensus sequence binding protein (ICSBP) is a transcription factor expressed mostly in the cells of the immune system. ICSBP belongs to the IFN regulatory factor (IRF) family, which also includes IRF-1, IRF-2, and the IFN-alpha-stimulated gene factor 3 gamma (ISGF3 gamma). We show here that ICSBP forms a complex with IRF-1 or IRF-2 both in vivo and in vitro and, in the presence or absence of the target DNA, with the IFN-stimulated response element (ISRE). Further, electrophoretic mobility shift assays show that this interaction greatly enhances the otherwise very low binding affinity of ICSBP to the ISRE. We show, on the other hand, that ICSBP inhibits binding of the IFN-alpha-stimulated gene factor 3 gamma to the ISRE. Through these interactions ICSBP is likely to exert complex modulatory functions in the regulation of IFN-stimulated genes.
Author List
Bovolenta C, Driggers PH, Marks MS, Medin JA, Politis AD, Vogel SN, Levy DE, Sakaguchi K, Appella E, Coligan JEAuthor
Jeffrey A. Medin PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Base SequenceCarrier Proteins
Cell Line
Consensus Sequence
DNA
DNA-Binding Proteins
Humans
Interferon Regulatory Factor-1
Interferon Regulatory Factor-2
Interferon Regulatory Factors
Interferon-Stimulated Gene Factor 3
Interferon-Stimulated Gene Factor 3, gamma Subunit
Molecular Sequence Data
Phosphoproteins
Precipitin Tests
Protein Binding
Repressor Proteins
Transcription Factors
