Faculty Collaboration Database

Inhibitory effects of merocyanine 540-mediated photodynamic therapy on cellular immune functions: A role in the prophylaxis of graft-versus-host disease? J Photochem Photobiol B 2015 Dec;153:153-63

Date

09/28/2015

Pubmed ID

26410039

Pubmed Central ID

PMC4674338

DOI

10.1016/j.jphotobiol.2015.09.012

Scopus ID

2-s2.0-84942320153 (requires institutional sign-in at Scopus site)   14 Citations

Abstract

Merocyanine 540-mediated photodynamic therapy (MC540-PDT) has been used in clinical trials for the purging of autologous hematopoietic stem cell grafts. When the same combinations of dye and light were applied to human peripheral blood lymphocytes, a broad range of T- and B-cell functions were impaired, prompting speculations about a potential role of MC540-PDT in the prophylaxis of graft-versus-host disease (GVHD). We here report on the effects of MC540-PDT on in vitro functions of murine lymphocytes as well as a preliminary evaluation of MC540-PDT for the prevention of GVHD in murine models of allogeneic bone marrow transplantation. Mixed lymphocyte reactions, proliferative responses to lectins, interleukin-2 and lipopolysaccharide, T-cell-mediated lysis, and NK activity were all inhibited by moderate doses of MC540-PDT. Whether MC540-PDT reduced the incidence and/or the severity of GVHD in murine models of allogeneic hematopoietic stem cell transplantation depended on the composition of the mismatched grafts and the intensity of the preparative regimen. MC540-PDT was only beneficial (i.e. reduced the incidence and/or severity of GVHD) when the spleen cell content of grafts was low and/or the radiation dose of the preparative regimen was not myeloablative, and, therefore, may have encouraged mixed chimerism.

Author List

Traul DL, Sieber F

Author

Fritz Sieber PhD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
B-Lymphocytes
Body Weight
Bone Marrow Cells
Bone Marrow Transplantation
Cell Proliferation
Cell Survival
Female
Graft vs Host Disease
Killer Cells, Natural
Light
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Photochemotherapy
Photosensitizing Agents
Pyrimidinones
T-Lymphocytes
Transplantation, Homologous