500-MHz proton homonuclear Overhauser evidence for additional base pair in the common arm of eukaryotic ribosomal 5S RNA: wheat germ. Biochemistry 1987 Mar 24;26(6):1578-85
Date
03/24/1987Pubmed ID
3109468DOI
10.1021/bi00380a014Scopus ID
2-s2.0-0023663351 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
A "common-arm" fragment from wheat germ (Triticum aestivum) 5S RNA has been produced by enzymatic cleavage with RNase T1 and sequenced via autoradiography of electrophoresis gels for the end-labeled fragments obtained by further RNase T1 partial digestion. The existence, base pair composition, and base pair sequence of the common arm are demonstrated for the first time by means of proton 500-MHz nuclear magnetic resonance. From Mg2+ titration, temperature variation, ring current calculations, sequence comparisons, and proton homonuclear Overhauser enhancement experiments, additional base pairs in the common arm of the eukaryotic 5S RNA secondary structure are detected. Two base pairs, G41 X C34 and A42 X U33 in the hairpin loop, could account for the lack of binding between the conserved GAAC segment of 5S RNA and the conserved Watson-Crick-complementary GT psi C segment of tRNAs.
Author List
Li SJ, Wu JJ, Marshall AGMESH terms used to index this publication - Major topics in bold
Base CompositionBase Sequence
Magnetic Resonance Spectroscopy
Molecular Weight
Nucleic Acid Conformation
Plants
RNA, Ribosomal
Ribonuclease T1
Triticum
