Increased expression of cyclooxygenase-2 and nitric oxide synthase-2 in human prostate cancer. Urol Res 2001 Feb;29(1):23-8
Date
04/20/2001Pubmed ID
11310211DOI
10.1007/s002400000148Scopus ID
2-s2.0-0035104125 (requires institutional sign-in at Scopus site) 136 CitationsAbstract
Cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase-2 (NOS-2) each have an important role in angiogenesis. The expression of these genes was investigated in human prostate cancer by immunohistochemistry, the expression of COX-1 and COX-2 being confirmed by mRNA analysis. Prostate cancer specimens from 12 patients were compared to control prostates from 13 patients operated on for bladder carcinoma. The intensity of COX-2 and NOS-2 immunostaining was significantly stronger in prostate cancer cells than in the non-malignant glandular epithelium of the control prostates. COX-2 and NOS-2 were clearly also expressed in the lesions of prostatic intraepithelial neoplasia (PIN) in control prostates. COX-2 was detected in the muscle fibres of the hyperplastic stroma of some control prostates. No significant difference was detected in COX-1 expression between control and cancer prostates. These results indicate that the expression of COX-2 and NOS-2 is elevated in prostatic adenocarcinoma and in PIN.
Author List
Uotila P, Valve E, Martikainen P, Nevalainen M, Nurmi M, Härkönen PMESH terms used to index this publication - Major topics in bold
AgedCyclooxygenase 1
Cyclooxygenase 2
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Isoenzymes
Male
Membrane Proteins
Middle Aged
Nitric Oxide Synthase
Nitric Oxide Synthase Type II
Prostaglandin-Endoperoxide Synthases
Prostatic Intraepithelial Neoplasia
Prostatic Neoplasms
RNA, Messenger