Faculty Collaboration Database

Nitric oxide production by monocytes in children with OSA and endothelial dysfunction. Clin Sci (Lond) 2014 Sep;127(5):323-30

Date

03/13/2014

Pubmed ID

24611870

DOI

10.1042/CS20130679

Scopus ID

2-s2.0-84903535320 (requires institutional sign-in at Scopus site)   17 Citations

Abstract

OSA (obstructive sleep apnoea) is associated with a higher risk for alterations in post-occlusive hyperaemia, an eNOS (endothelial NO synthase)-dependent endothelial response. However, since not all children manifest endothelial dysfunction, we hypothesized that differences in circulating monocyte subsets and NO production may underlie the vascular phenotype in paediatric OSA. Matched pre-pubertal children with OSA with abnormal endothelial function (OSAab) and with normal endothelial function (OSAn), and controls (CO) were recruited. Peripheral blood mononuclear cells were subtyped into CD14+ and CD16+ cells, and NO production was assessed using flow cytometry. Endothelial dysfunction was defined as Tmax (time to reach maximal reperfusion)>45 s by laser Doppler flowmetry. A total of 11 OSAab, 12 OSAn and 12 CO-matched children completed the study. The OSAab group had increased CD16+ and decreased CD14+ cell numbers. They also had increased CX3CR1 (CX3C chemokine receptor 1) expression in CD16+ monocytes (P<0.01). Furthermore, monocytes from the OSAab group exhibited overall reduced NO production (787±71 compared with 1226±229 and 1089±116 median fluorescence intensity in the OSAn group and CO children respectively; P<0.01). Significant bivariate associations emerged between NO production, monocyte subsets, CX3CR1 in CD16+ monocytes, the CD14+/CD16+ ratio and Tmax. Thus OSA in children is associated with increased numbers of pro-inflammatory monocytes and reduced NO production in circulating monocytes that are closely associated with endothelial function.

Author List

Kheirandish-Gozal L, Wang Y, Duggan RC, Harshan Vardhan S, Tan HL, Molero Ramirez H, Khalyfa A, Bhattacharjee R, Bandla HP, Gozal D

Author

Hari Bandla MD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Child
Child, Preschool
Endothelium, Vascular
Humans
Leukocytes, Mononuclear
Nitric Oxide
Sleep Apnea, Obstructive