Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Assessment of degradation and ubiquitination of CXCR4, a GPCR regulated by EGFR family members. Methods Mol Biol 2006;327:139-45

Date

06/20/2006

Pubmed ID

16780218

DOI

10.1385/1-59745-012-X:139

Scopus ID

2-s2.0-33745969459   7 Citations

Abstract

The ErbB2/HER2 receptors are aberrantly expressed in certain mammary epithelial cancers. A recent study has shown that in a subset of these breast cancers, the HER2 receptors contribute to increased cell surface levels of the chemokine receptor CXCR4, which in turn results in increased metastasis of the breast cancers. Therefore, information concerning the mechanisms regulating CXCR4 receptors levels is essential to our understanding of its role in cancer cell metastasis. CXCR4 is a member of the G protein-coupled receptor (GPCR) family, and herein we describe methods to monitor the ubiquitination, degradation, and downregulation of CXCR4.

Author List

Marchese A

Author

Adriano Marchese PhD Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

ErbB Receptors
HeLa Cells
Humans
Protein Processing, Post-Translational
Receptors, CXCR4
Ubiquitin