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Ubiquitination of G-protein-coupled receptors. Methods Mol Biol 2004;259:299-305

Date

07/15/2004

Pubmed ID

15250500

DOI

10.1385/1-59259-754-8:299

Scopus ID

2-s2.0-4043140225   12 Citations

Abstract

In this chapter we describe methods for detecting the ubiquitination state of G-protein-coupled receptors (GPCRs). This involves coexpression of a GPCR with an epitope-tagged ubiquitin construct in a heterologous expression system. Modification by ubiquitin of the GPCR resulting from agonist activation is detected by immunoprecipation and subsequent immunoblotting for the epitope-tagged ubiquitin. We use here the chemokine receptor CXCR4 as the model receptor; however, this could be easily modified to detect the ubiquitination state of any GPCR.

Author List

Marchese A, Benovic JL

Author

Adriano Marchese PhD Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Humans
Receptors, G-Protein-Coupled
Ubiquitin