Ubiquitination of G-protein-coupled receptors. Methods Mol Biol 2004;259:299-305
Date
07/15/2004Pubmed ID
15250500DOI
10.1385/1-59259-754-8:299Scopus ID
2-s2.0-4043140225 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
In this chapter we describe methods for detecting the ubiquitination state of G-protein-coupled receptors (GPCRs). This involves coexpression of a GPCR with an epitope-tagged ubiquitin construct in a heterologous expression system. Modification by ubiquitin of the GPCR resulting from agonist activation is detected by immunoprecipation and subsequent immunoblotting for the epitope-tagged ubiquitin. We use here the chemokine receptor CXCR4 as the model receptor; however, this could be easily modified to detect the ubiquitination state of any GPCR.
Author List
Marchese A, Benovic JLAuthor
Adriano Marchese PhD Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
HumansReceptors, G-Protein-Coupled
Ubiquitin