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Pupillary effects of neurotensin: structure-activity relationships. Neuropeptides 1985 Dec;6(6):561-8

Date

12/01/1985

Pubmed ID

2867486

DOI

10.1016/0143-4179(85)90119-2

Scopus ID

2-s2.0-0022203576   8 Citations

Abstract

We have previously reported that intracameral (I.C.) administration of neurotensin (NT) potently induces a time- and dose-dependent miosis in rabbits. This study was designed to determine structure-function relationships for NT-induced miosis. NT and twelve different fragments and analogs of NT, and the structurally-unrelated peptides beta-endorphin (beta-end), somatostatin (SRIF) and thyrotropin-releasing hormone (TRH) were tested in a dose equimolar to 30 micrograms of NT for their effects on pupillary diameter (PD) in rabbits. In confirmation of previous findings, NT produced significant miosis. Followed in order of duration of effect were D-Trp11-NT, D-Tyr11-NT, the N-terminal fragment NT1-12, [Gln4] - NT and NMe-NT. The N-terminal fragment NT1-8, D-Arg8-NT, and D-Phe11-NT were weakly active. In addition, the initial N-terminal fragment NT1-6 and the C-terminal fragments NT8-13 and NT9-13 did not affect PD. D-Pro10-NT, beta-end, SRIF, and TRH were totally ineffective. The results of this investigation contribute to support a role for NT on regulation of pupillary function, and suggest that the midportion of NT appears to be critical for the expression of NT-induced miosis.

Author List

Hernandez DE, Simons KB, Spampinato D, Rioux F, St-Pierre S

Author

Kenneth B. Simons MD Sr Associate Dean, Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Anterior Chamber
Endorphins
Injections
Neurotensin
Pupil
Rabbits
Somatostatin
Structure-Activity Relationship
Thyrotropin-Releasing Hormone
beta-Endorphin