Arginase-1 is expressed exclusively by infiltrating myeloid cells in CNS injury and disease. Brain Behav Immun 2016 Aug;56:61-7
Date
04/30/2016Pubmed ID
27126514DOI
10.1016/j.bbi.2016.04.013Scopus ID
2-s2.0-84974663075 (requires institutional sign-in at Scopus site) 51 CitationsAbstract
Resident microglia and infiltrating myeloid cells play important roles in the onset, propagation, and resolution of inflammation in central nervous system (CNS) injury and disease. Identifying cell type-specific mechanisms will help to appropriately target interventions for tissue repair. Arginase-1 (Arg-1) is a well characterised modulator of tissue repair and its expression correlates with recovery after CNS injury. Here we assessed the cellular localisation of Arg-1 in two models of CNS damage. Using microglia specific antibodies, P2ry12 and Fc receptor-like S (FCRLS), we show the LysM-EGFP reporter mouse is an excellent model to distinguish infiltrating myeloid cells from resident microglia. We show that Arg-1 is expressed exclusively in infiltrating myeloid cells but not microglia in models of spinal cord injury (SCI) and experimental autoimmune encephalomyelitis (EAE). Our in vitro studies suggest that factors in the CNS environment prevent expression of Arg-1 in microglia in vivo. This work suggests different functional roles for these cells in CNS injury and repair and shows that such repair pathways can be switched on in infiltrating myeloid cells in pro-inflammatory environments.
Author List
Greenhalgh AD, Passos Dos Santos R, Zarruk JG, Salmon CK, Kroner A, David SAuthor
Antje Kroner-Milsch MD, PhD Associate Professor in the Neurosurgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsArginase
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental
Female
Green Fluorescent Proteins
Inflammation
Mice
Mice, Inbred C57BL
Microglia
Muramidase
Myeloid Cells
Spinal Cord Injuries
