Medical College of Wisconsin
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Complexes of non-cationic liposomes and histone H1 mediate efficient transfection of DNA without encapsulation. Biochim Biophys Acta 1996 Oct 02;1284(1):47-55

Date

10/02/1996

Pubmed ID

8865814

DOI

10.1016/0005-2736(96)00106-x

Abstract

Transfection competent complexes were assembled using a three component system. The constituents of the basic system were plasmid DNA, cationic DNA binding protein (NLS-H1) and anionic liposomes (dioleoyl phosphatidylethanolamine (DOPE) or phosphatidylserine (PS)). In contrast to cationic liposome/DNA binary complexes, all of the DNA in these ternary complexes was sensitive to DNase I degradation and ethidium bromide intercalation. Transmission electron microscopy revealed that these ternary complexes formed unique structures in which the DNA was located either on the outside of individual liposomes or bridging two or more liposomes. This provides evidence that plasmid DNA encapsulation is not essential for transfection competency.

Author List

Hagstrom JE, Sebestyen MG, Budker V, Ludtke JJ, Fritz JD, Wolff JA

Author

Jeffery Duane Fritz PhD Associate Professor in the Medical School Regional Campuses department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

3T3 Cells
Animals
DNA
DNA, Superhelical
Drug Carriers
Gene Transfer Techniques
Genetic Therapy
Histones
Liposomes
Mice
Microscopy, Electron
Plasmids
Transfection