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Gene transfer into mammalian cells using histone-condensed plasmid DNA. Hum Gene Ther 1996 Aug 01;7(12):1395-404

Date

08/01/1996

Pubmed ID

8844198

DOI

10.1089/hum.1996.7.12-1395

Abstract

A recombinant histone (NLS-H1) containing both the SV40 large T antigen nuclear localization signal and the carboxy-terminal domain of human histone H1(0) was produced in bacteria. NLS-H1-plasmid DNA complexes, in the presence of chloroquine, mediated reporter gene transfer into cultured cells with similar efficiencies as plasmid DNA-cationic lipid (lipofectin) complexes. NIH-3T3 or COS-7 cells transfected with NLS-H1-plasmid DNA-lipofectin complexes expressed at least 20 times more luciferase or had at least 2.5 times more beta-galactosidase-positive cells than those transfected with plasmid DNA-lipofectin complexes. Foreign gene expression was also improved by other DNA-binding proteins and cationic lipid formulations, yet the greatest enhancement was obtained with complexes containing either NLS-H1 or calf thymus histone H1. Histone H1-plasmid DNA-lipofectin complexes were internalized by a greater number of cells than plasmid DNA-lipofectin complexes.

Author List

Fritz JD, Herweijer H, Zhang G, Wolff JA

Author

Jeffery Duane Fritz PhD Associate Professor in the Medical School Regional Campuses department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

3T3 Cells
Animals
Antigens, Polyomavirus Transforming
COS Cells
Cattle
Cell Line, Transformed
Cells, Cultured
Chloroquine
DNA, Recombinant
Gene Expression
Gene Transfer Techniques
Genes, Reporter
Genetic Vectors
HeLa Cells
Histones
Humans
Kidney
Luciferases
Mice
Phosphatidylethanolamines
Recombinant Fusion Proteins