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Effect of vitamin E on FMLP-induced activation of rabbit polymorphonuclear leukocytes. Inflammation 1987 Sep;11(3):309-21

Date

09/01/1987

Pubmed ID

2820879

DOI

10.1007/BF00915835

Abstract

Granulocytes of vitamin E-treated rabbits were compared to granulocytes from placebo-treated rabbits. Granulocytes were isolated from rabbit peripheral blood by a new method employing Percoll and gelatin sedimentation. Vitamin E-treated cells showed less adherence to rabbit aortic endothelium when stimulated with FMLP. FMLP receptor numbers and affinity were not significantly different. Resting cell surface and baseline transmembrane potential were similar in both cell types. Decrease in cell surface potential with FMLP was comparable in vitamin E- and placebo-treated cells. Vitamin E-treated PMN depolarized more and hyperpolarized more rapidly than placebo cells. Thus vitamin E-treated PMNs show differences in the early events of PMN activation. These may contribute to the lower stimulated adherence observed with vitamin E-treated cells.

Author List

Weisman SJ, Lafuze JE, Haak RA, Baehner RL

Author

Steven J. Weisman MD Professor in the Anesthesiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Cell Adhesion
Cell Separation
Endothelium, Vascular
Male
Membrane Potentials
N-Formylmethionine Leucyl-Phenylalanine
Neutrophils
Rabbits
Receptors, Cell Surface
Vitamin E
jenkins-FCD Prod-484 8aa07fc50b7f6d102f3dda2f4c7056ff84294d1d