Singlet oxygen intermediacy in the photodynamic action of membrane-bound hematoporphyrin derivative. Cancer Lett 1987 Jun;35(3):295-302
Date
06/01/1987Pubmed ID
2954632DOI
10.1016/0304-3835(87)90131-5Scopus ID
2-s2.0-0023189023 (requires institutional sign-in at Scopus site) 64 CitationsAbstract
The cell-damaging photochemistry of hematoporphyrin derivative (HPD) has been investigated using isolated erythrocyte membranes as a test system. Irradiation of membranes in the presence of the tumor-localizing fraction of HPD resulted in formation of singlet molecular oxygen (1O2) as measured by the phosphorescence at 1268 nm. The authentic product of 1O2 attack on cholesterol, 3 beta-hydroxy-5 alpha-cholest-6-ene-5-hydroperoxide, was identified in this system. Relatively insignificant amounts of free radical-derived hydroperoxides were detected. These results suggest that 1O2 plays a major role in the HPD-sensitized photokilling of tumor cells in vivo.
Author List
Thomas JP, Hall RD, Girotti AWAuthors
Albert W. Girotti PhD Adjunct Professor in the Biochemistry department at Medical College of WisconsinJames P. Thomas MD, PhD Professor in the Medicine department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
CholesterolChromatography, Thin Layer
Erythrocyte Membrane
Hematoporphyrin Derivative
Hematoporphyrins
Humans
Luminescent Measurements
Oxygen
Photochemistry
Radiation-Sensitizing Agents