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Singlet oxygen intermediacy in the photodynamic action of membrane-bound hematoporphyrin derivative. Cancer Lett 1987 Jun;35(3):295-302

Date

06/01/1987

Pubmed ID

2954632

DOI

10.1016/0304-3835(87)90131-5

Scopus ID

2-s2.0-0023189023 (requires institutional sign-in at Scopus site)   64 Citations

Abstract

The cell-damaging photochemistry of hematoporphyrin derivative (HPD) has been investigated using isolated erythrocyte membranes as a test system. Irradiation of membranes in the presence of the tumor-localizing fraction of HPD resulted in formation of singlet molecular oxygen (1O2) as measured by the phosphorescence at 1268 nm. The authentic product of 1O2 attack on cholesterol, 3 beta-hydroxy-5 alpha-cholest-6-ene-5-hydroperoxide, was identified in this system. Relatively insignificant amounts of free radical-derived hydroperoxides were detected. These results suggest that 1O2 plays a major role in the HPD-sensitized photokilling of tumor cells in vivo.

Author List

Thomas JP, Hall RD, Girotti AW

Authors

Albert W. Girotti PhD Adjunct Professor in the Biochemistry department at Medical College of Wisconsin
James P. Thomas MD, PhD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Cholesterol
Chromatography, Thin Layer
Erythrocyte Membrane
Hematoporphyrin Derivative
Hematoporphyrins
Humans
Luminescent Measurements
Oxygen
Photochemistry
Radiation-Sensitizing Agents