A systematic analysis of neonatal mouse heart regeneration after apical resection. J Mol Cell Cardiol 2015 Feb;79:315-8
Date
12/24/2014Pubmed ID
25533939Pubmed Central ID
PMC4302033DOI
10.1016/j.yjmcc.2014.12.011Scopus ID
2-s2.0-84920973650 (requires institutional sign-in at Scopus site) 87 CitationsAbstract
The finding that neonatal mice are able to regenerate myocardium after apical resection has recently been questioned. We determined if heart regeneration is influenced by the size of cardiac resection and whether surgical retraction of the ventricular apex results in an increase in cardiomyocyte cell cycle activity. We performed moderate or large apical ventricular resections on neonatal mice and quantified scar infiltration into the left ventricular wall at 21 days post-surgery. Moderately resected hearts had 15±2% of the wall infiltrated by a collagen scar; significantly greater scar infiltration (23±4%) was observed in hearts with large resections. Resected hearts had higher levels of cardiomyocyte cell cycle activity relative to sham hearts. Surgically retracting the ventricle often resulted in fibrosis and induced cardiomyocyte cell cycle activity that were comparable to that of resected hearts. We conclude that apical resection in neonatal mice induces cardiomyocyte cell cycle activity and neomyogenesis, although scarring can occur. Surgical technique and definition of approach to assessing the extent of regeneration are both critical when using the neonatal mouse apical resection model.
Author List
Bryant DM, O'Meara CC, Ho NN, Gannon J, Cai L, Lee RTAuthor
Caitlin C. O'Meara PhD Associate Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Newborn
Cardiac Surgical Procedures
Cell Cycle
Fibrosis
Heart
Heart Ventricles
Mice
Myocardium
Myocytes, Cardiac
Regeneration