Synthesis by cultured human umbilical vein endothelial cells of two proteins structurally and immunologically related to platelet membrane glycoproteins IIb and IIIa. J Cell Biol 1986 Jul;103(1):81-6
Date
07/01/1986Pubmed ID
2941443Pubmed Central ID
PMC2113805DOI
10.1083/jcb.103.1.81Scopus ID
2-s2.0-0022474387 (requires institutional sign-in at Scopus site) 28 CitationsAbstract
Human platelets participate in a number of adhesive interactions, including binding to exposed subendothelium after vascular injury, and platelet-platelet cohesion to form large aggregates. Platelet membrane glycoproteins (GP) IIb and IIIa constitute a receptor for fibrinogen that, together with fibrinogen and calcium, is largely responsible for mediating the formation of the primary hemostatic plug. Using highly specific polyclonal and monoclonal antibodies as probes, we could detect the presence of both of these glycoproteins in cultured human umbilical vein endothelial cells. Western-blot analysis showed that the endothelial cell analogues were similar in size to their platelet counterparts, and were present in cells that had been in culture for over 2 mo. Metabolic labeling of endothelium with [35S]methionine demonstrated that both GPIIb and GPIIIa were actively synthesized in culture. Using the technique of crossed immunoelectrophoresis, evidence was obtained that the endothelial cell forms of GPIIb and GPIIIa may exist complexed to one another after solubilization in Triton X-100. The presence of GPIIb-IIIa analogues in cultured endothelial cells may provide an opportunity to examine the structure, function, and synthesis of these two membrane glycoproteins, as well as provide a source of genetic material with which to begin detailed molecular genetic studies.
Author List
Newman PJ, Kawai Y, Montgomery RR, Kunicki TJAuthor
Robert R. Montgomery MD Adjunct Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Antibodies, MonoclonalBlood Platelets
Cell Aggregation
Cells, Cultured
Cross Reactions
Endothelium
Glycoproteins
Humans
Immunoelectrophoresis, Two-Dimensional
Membrane Proteins
Molecular Weight
Platelet Membrane Glycoproteins
Umbilical Veins