CD82 Is a Marker for Prospective Isolation of Human Muscle Satellite Cells and Is Linked to Muscular Dystrophies. Cell Stem Cell 2016 Dec 01;19(6):800-807
Date
09/20/2016Pubmed ID
27641304Pubmed Central ID
PMC5135584DOI
10.1016/j.stem.2016.08.006Scopus ID
2-s2.0-84994721676 (requires institutional sign-in at Scopus site) 81 CitationsAbstract
Cell-surface markers for prospective isolation of stem cells from human skeletal muscle have been difficult to identify. Such markers would be powerful tools for studying satellite cell function during homeostasis and in pathogenesis of diseases such as muscular dystrophies. In this study, we show that the tetraspanin KAI/CD82 is an excellent marker for prospectively isolating stem cells from human fetal and adult skeletal muscle. Human CD82+ muscle cells robustly engraft into a mouse model of muscular dystrophy. shRNA knockdown of CD82 in myogenic cells reduces myoblast proliferation, suggesting it is functionally involved in muscle homeostasis. CD82 physically interacts with alpha7beta1 integrin (α7β1-ITG) and with α-sarcoglycan, a member of the Dystrophin-Associated Glycoprotein Complex (DAPC), both of which have been linked to muscular dystrophies. Consistently, CD82 expression is decreased in Duchenne muscular dystrophy patients. Together, these findings suggest that CD82 function may be important for muscle stem cell function in muscular disorders.
Author List
Alexander MS, Rozkalne A, Colletta A, Spinazzola JM, Johnson S, Rahimov F, Meng H, Lawlor MW, Estrella E, Kunkel LM, Gussoni EAuthor
Michael W. Lawlor MD, PhD Adjunct Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultAnimals
Antigens, CD
Biomarkers
CD146 Antigen
Cell Separation
HEK293 Cells
Humans
Immunoprecipitation
Integrin alpha Chains
Kangai-1 Protein
Mice, SCID
Muscular Dystrophies
Muscular Dystrophy, Animal
Sarcoglycans
Satellite Cells, Skeletal Muscle