Faculty Collaboration Database

Platelet rich plasma extract promotes angiogenesis through the angiopoietin1-Tie2 pathway. Microvasc Res 2013 Sep;89:15-24

Date

05/11/2013

Pubmed ID

23660186

DOI

10.1016/j.mvr.2013.04.008

Scopus ID

2-s2.0-84883172376 (requires institutional sign-in at Scopus site)   103 Citations

Abstract

Development and regeneration of tissues and organs require precise coordination among endothelial, epithelial and mesenchymal morphogenesis. Angiogenesis plays key roles in normal development, wound healing, recovery from ischemic disease, and organ regeneration. It has been recognized that the combination of various angiogenic factors in an appropriate physiological ratio is critical for long-term functional blood vessel formation. Here we show that mouse soluble platelet-rich-plasma (PRP) extract, which includes abundant angiopoetin-1 (Ang1) and other angiogenic factors, stimulates endothelial cell growth, migration and differentiation in cultured human dermal microvascular endothelial cells in vitro and neonatal mouse retinal angiogenesis in vivo. Mouse platelet rich fibrin (PRF) matrix, the three-dimensional fibrin matrix that releases angiogenic factors with similar concentrations and proportions to the PRP extract, also recapitulates robust angiogenesis inside the matrix when implanted subcutaneously on the living mouse. Inhibition of Ang1-Tie2 signaling suppresses PRP extract-induced angiogenesis in vitro and angiogenic ability of the PRF matrix in vivo. Since human PRP extract and PRF matrix can be prepared from autologous peripheral blood, our findings may lead to the development of novel therapeutic interventions for various angiogenesis-related diseases as well as to the improvement of strategies for tissue engineering and organ regeneration.

Author List

Mammoto T, Jiang A, Jiang E, Mammoto A

Authors

Akiko Mammoto MD, PhD Professor in the Pediatrics department at Medical College of Wisconsin
Tadanori Mammoto PhD Associate Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Angiopoietin-1
Animals
Cell Proliferation
Endothelial Cells
Gene Expression Regulation, Developmental
Humans
Mice
Mice, Inbred C57BL
Microcirculation
Microscopy, Fluorescence
Neovascularization, Physiologic
Platelet-Rich Plasma
RNA Interference
Receptor, TIE-2
Regeneration
Retina
Retinal Vessels