Determination of parthenolide in selected feverfew products by liquid chromatography. J AOAC Int 2000;83(4):789-92
Date
09/20/2000Pubmed ID
10995104Scopus ID
2-s2.0-0034219691 (requires institutional sign-in at Scopus site) 17 CitationsAbstract
The migraine prophylactic herb feverfew (Tanacetum parthenium L.) is marketed in the United States in a variety of forms and compositions. Although its therapeutic efficacy is still uncertain, the sesquiterpene lactone parthenolide is the constituent recommended to be measured for quality control of feverfew preparations. A validated liquid chromatographic method was developed and used to estimate parthenolide in a number of U.S. feverfew market products formulated as capsules, tablets, or crude powder. The method uses a Lichrosphere 5 C18 column, a mobile phase consisting of 50 mM NaH2PO4 in H2O (solvent A), and CH3CN-MeOH (90 + 10, v/v; solvent B). Elution was run at a flow rate of 1.0 mL/min with a linear gradient of 50-15% A in B over 20 min and UV detection at 210 nm. The correlation coefficient for the calibration curve was 0.9999 over the range of 0.00-0.400 mg/mL. Overall recovery of parthenolide was 103.1%.
Author List
Abourashed EA, Khan IA, Abourashed EA, Khan IAAuthor
Ehab A. Abourashed PhD Professor in the School of Pharmacy Administration department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Anti-Inflammatory Agents, Non-SteroidalChromatography, Liquid
Drug Costs
Drug Labeling
Humans
Linear Models
Magnoliopsida
Migraine Disorders
Pharmaceutical Preparations
Plants, Medicinal
Quality Control
Sensitivity and Specificity
Sesquiterpenes
Solutions
Spectrophotometry, Ultraviolet