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Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection. Stem Cell Reports 2017 03 14;8(3):491-499

Date

02/28/2017

Pubmed ID

28238794

Pubmed Central ID

PMC5355643

DOI

10.1016/j.stemcr.2017.01.021

Scopus ID

2-s2.0-85013648619   15 Citations

Abstract

Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models.

Author List

Mitzelfelt KA, McDermott-Roe C, Grzybowski MN, Marquez M, Kuo CT, Riedel M, Lai S, Choi MJ, Kolander KD, Helbling D, Dimmock DP, Battle MA, Jou CJ, Tristani-Firouzi M, Verbsky JW, Benjamin IJ, Geurts AM

Authors

Michele A. Battle PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin
Ivor J. Benjamin MD Center Director, Professor in the Medicine department at Medical College of Wisconsin
Aron Geurts PhD Associate Professor in the Physiology department at Medical College of Wisconsin
James Verbsky MD, PhD Associate Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

CRISPR-Cas Systems
Cell Line
DNA End-Joining Repair
Gene Editing
Gene Knock-In Techniques
Gene Targeting
Genetic Vectors
Genome, Human
High-Throughput Nucleotide Sequencing
Humans
Induced Pluripotent Stem Cells
Recombinational DNA Repair
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a