Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection. Stem Cell Reports 2017 Mar 14;8(3):491-499
Date
02/28/2017Pubmed ID
28238794Pubmed Central ID
PMC5355643DOI
10.1016/j.stemcr.2017.01.021Scopus ID
2-s2.0-85013648619 (requires institutional sign-in at Scopus site) 24 CitationsAbstract
Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models.
Author List
Mitzelfelt KA, McDermott-Roe C, Grzybowski MN, Marquez M, Kuo CT, Riedel M, Lai S, Choi MJ, Kolander KD, Helbling D, Dimmock DP, Battle MA, Jou CJ, Tristani-Firouzi M, Verbsky JW, Benjamin IJ, Geurts AMAuthors
Ivor J. Benjamin MD Center Director, Professor in the Medicine department at Medical College of WisconsinAron Geurts PhD Professor in the Physiology department at Medical College of Wisconsin
James Verbsky MD, PhD Professor in the Pediatrics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
CRISPR-Cas SystemsCell Line
DNA End-Joining Repair
Gene Editing
Gene Knock-In Techniques
Gene Targeting
Genetic Vectors
Genome, Human
High-Throughput Nucleotide Sequencing
Humans
Induced Pluripotent Stem Cells
Recombinational DNA Repair
