Healing a Heart Through Genetic Intervention. Circ Res 2016 Mar 18;118(6):920-2
Date
03/19/2016Pubmed ID
26987913Pubmed Central ID
PMC4801122DOI
10.1161/CIRCRESAHA.116.308468Scopus ID
2-s2.0-84961775468 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
A recent manuscript from Eric Olson’s group outlines the development of an important new tool that should catalyze the cardiovascular community’s ability to create new animal models containing genetically engineered genes and proteins specifically in the cardiomyocyte (CM) population. Olson and colleagues utilized a CM-specific promoter to express a critical component of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas)9 genomic editing system. The α myosin heavy chain gene (myh6) promoter was used to drive high levels of CM-specific Cas9 expression. Subsequently, Adeno-Associated Virus (AAV) was used to deliver single-guide RNA (sgRNA) against the myh6 locus and they were able to show efficient gene editing at the locus, establishing proof of principle for what the authors term “cardioediting,” a strategy for revising a specific locus in the CMs at any time post birth.
Author List
James J, Robbins JMESH terms used to index this publication - Major topics in bold
AgingAnimals
CRISPR-Cas Systems
Gene Deletion
Myocardium
