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Multicenter Evaluation of the Bruker MALDI Biotyper CA System for the Identification of Clinically Important Bacteria and Yeasts. Am J Clin Pathol 2017 Jun 01;147(6):623-631

Date

05/16/2017

Pubmed ID

28505220

DOI

10.1093/ajcp/aqw225

Scopus ID

2-s2.0-85031705467 (requires institutional sign-in at Scopus site)   29 Citations

Abstract

OBJECTIVES: A report on the multicenter evaluation of the Bruker MALDI Biotyper CA System (MBT-CA; Bruker Daltonics, Billerica, MA) for the identification of clinically important bacteria and yeasts.

METHODS: In total, 4,399 isolates of medically important bacteria and yeasts were assessed in the MBT-CA. These included 2,262 aerobic gram-positive (AGP) bacteria, 792 aerobic gram-negative (AGN) bacteria 530 anaerobic (AnA) bacteria, and 815 yeasts (YSTs). Three processing methods were assesed.

RESULTS: Overall, 98.4% (4,329/4,399) of all bacterial and yeast isolates were correctly identified to the genus and species/species complex level, and 95.7% of isolates were identified with a high degree of confidence. The percentage correctly identified and the percentage identified correctly with a high level of confidence, respectively, were as follows: AGP bacteria (98.6%/96.5%), AGN bacteria (98.5%/96.8%), AnA bacteria (98.5%/97.4%), and YSTs (97.8%/87.6%). The extended direct transfer method was only minimally superior to the direct transfer method for bacteria (89.9% vs 86.8%, respectively) but significantly superior for yeast isolates (74.0% vs 48.9%, respectively).

CONCLUSIONS: The Bruker MALDI Biotyper CA System accurately identifies most clinically important bacteria and yeasts and has optional processing methods to improve isolate characterization.

Author List

Wilson DA, Young S, Timm K, Novak-Weekley S, Marlowe EM, Madisen N, Lillie JL, Ledeboer NA, Smith R, Hyke J, Griego-Fullbright C, Jim P, Granato PA, Faron ML, Cumpio J, Buchan BW, Procop GW

Authors

Blake W. Buchan PhD Professor in the Pathology and Laboratory Medicine department at Medical College of Wisconsin
Nathan A. Ledeboer PhD Chief, Professor in the Pathology and Laboratory Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Bacteria
Bacterial Typing Techniques
Humans
Mycological Typing Techniques
Reproducibility of Results
Software
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Yeasts